DNA电泳基本参数 | |||
分离范围(bp) | 琼脂糖浓度 | 分离范围(bp) | PAGE浓度 |
1,000~30,000 | 0.50% | 100~1,000 | 3.50% |
800~12,000 | 0.70% | 80~500 | 5.00% |
500~10,000 | 1.00% | 60~400 | 8.00% |
400~7,000 | 1.20% | 40~200 | 12.00% |
200~4,000 | 1.50% | 5~100 | 20.00% |
50~2,000 | 2.00% | ||
PAGE浓度 | (相当核苷酸片段大小,bp) | ||
溴酚蓝 | 二甲苯青 | ||
3.50% | 100 | 460 | |
5.00% | 65 | 260 | |
8.00% | 45 | 160 | |
12.00% | 20 | 70 | |
15.00% | 15 | 60 | |
20.00% | 12 | 45 |
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